| Autor: |
Puls, Jan-Samuel, Winnerling, Benjamin, Power, Jeffrey J, Krüger, Annika M, Brajtenbach, Dominik, Johnson, Matthew, Bilici, Kevser, Camus, Laura, Fließwasser, Thomas, Schneider, Tanja, Sahl, Hans-Georg, Ghosal, Debnath, Kubitscheck, Ulrich, Heilbronner, Simon, Grein, Fabian |
| Zdroj: |
The ISME Journal; January 2024, Vol. 18 Issue: 1 |
| Abstrakt: |
Many bacteria produce antimicrobial compounds such as lantibiotics to gain advantage in the competitive natural environments of microbiomes. Epilancins constitute an until now underexplored family of lantibiotics with an unknown ecological role and unresolved mode of action. We discovered production of an epilancin in the nasal isolate Staphylococcus epidermidisA37. Using bioinformatic tools, we found that epilancins are frequently encoded within staphylococcal genomes, highlighting their ecological relevance. We demonstrate that production of epilancin A37 contributes to Staphylococcus epidermidiscompetition specifically against natural corynebacterial competitors. Combining microbiological approaches with quantitative in vivoand in vitrofluorescence microscopy and cryo-electron tomography, we show that A37 enters the corynebacterial cytoplasm through a partially transmembrane-potential-driven uptake without impairing the cell membrane function. Upon intracellular aggregation, A37 induces the formation of intracellular membrane vesicles, which are heavily loaded with the compound and are essential for the antibacterial activity of the epilancin. Our work sheds light on the ecological role of epilancins for staphylococci mediated by a mode of action previously unknown for lantibiotics. |
| Databáze: |
Supplemental Index |
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