Construction of a Yeast Artificial Chromosome Contig Spanning the Pseudoautosomal Region and Isolation of 25 New Sequence-Tagged Sites

Autor: Slim, Rima, Paslier, Denis Le, Compain, Sylvie, Levilliers, Jacqueline, Ougen, Pierre, Billault, Alain, Donohue, Susan J., Klein, David C., Mintz, Liat, Bernheim, Alain, Cohen, Daniel, Weissenbach, Jean, Petit, Christine
Zdroj: Genomics; June 1993, Vol. 16 Issue: 3 p691-697, 7p
Abstrakt: Thirty-one yeast artificial chromosomes (YACs) from the human pseudoautosomal region were identified by a combination of sequence-tagged site (STS) screenings and colony hybridizations, using a subtelomeric interspersed repetitive element mapping predominantly to the pseudoautosomal region. Twenty-five new pseudoautosomal STSs were generated, of which 4 detected restriction fragment length polymorphisms. A total of 33 STSs were used to assemble the 31 YACs into a single contiguous set of overlapping DNA fragments spanning at least 2.3 megabases of the pseudoautosomal region. In addition, four pseudoautosomal genes including hydroxyindole O-methyltransferase have been positioned on this set of fragments. Copyright 1993, 1999 Academic Press
Databáze: Supplemental Index