| Autor: |
Lyu, Yidan, Huang, Hao, Su, Yuyan, Ying, Binbin, Liu, Wen-Che, Dong, Kairu, Du, Ningjie, Langer, Robert S., Gu, Zhen, Nan, Kewang |
| Zdroj: |
Matter; April 2024, Vol. 7 Issue: 4 p1440-1465, 26p |
| Abstrakt: |
Engineered bacteria are increasingly recognized as sustained and intelligent sources for sensing and therapeutics due to their unique capabilities such as in situmultiplication, tissue targeting, and genetic editability. However, the clinical applications of these living agents are hindered by the ineffective immunoisolation, residency, and removal against the complex and dynamic in vivoenvironment. Existing approaches focus on surface decoration and encapsulation of engineered bacteria, or “microencapsulation,” but there are limits to what can be achieved with modifications of bacteria themselves. An emerging strategy combines millimeter- to centimeter-scale engineered devices and systems with bacteria, or “macroencapsulation,” offering unique advantages such as extending the in vivolifetime and engraftment of bacteria, enhancing immunoisolation, and enabling real-time signal readouts via wireless electronic technologies. In this review, the design rationales for macroencapsulated bacteria toward in vivoapplications are discussed, and examples in bacterial devices for transdermal and oral applications are highlighted. Since the gastrointestinal tract represents a major site for engineered bacteria, we also summarize and compare various strategies for synthetic engraftment of orally administered encapsulated bacteria. |
| Databáze: |
Supplemental Index |
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