| Autor: |
Nicolson, A.G., Haites, N.E., Mckay, N.G., Wilson, H.M., Macleod, A.M., Benjamin, N. |
| Zdroj: |
Biochemical and Biophysical Research Communications; June 1993, Vol. 193 Issue: 3 p1269-1274, 6p |
| Abstrakt: |
Synthesis of nitric oxide (NO) has been implicated in the development of glomerulonephritis in animal models of the disease. Rat mesangial cells can he stimulated to express an inducible form of NO synthase (NOS) in vitro. Little is known however, about the pathway of induction in human mesangial cells. Here, we report that human mesangial cells require multiple cytokines, unlike rat mesangial cells which require only single stimulants, to produce NO. Our experiments suggest that both interleukin-l beta (IL-1β) and interferon gamma (IFN-γ) must he present together to elicit a response whilst tumour necrosis factor alpha (TNF-α) augments this. The production of nitrite, a stable end product of NO metabolism, was inhibited by NG-monomethyl-L-arginine (L-NMMA), L-nitro-arginine-methyl-ester (L-NAME), cycloheximide and the glucocorticoid dexamethasone. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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