Engineering of ketocarotenoid biosynthetic pathway in Chlamydomonas reinhardtiithrough exogenous gene expression

Autor: Sharma, Arti, Nawkarkar, Prachi, Kapase, Vikas U., Chhabra, Meenu, Kumar, Shashi
Zdroj: Systems Microbiology and Biomanufacturing; 20240101, Issue: Preprints p1-13, 13p
Abstrakt: Graphical abstract: Graphical abstract for the study carried out which represents the in silicoplasmid vector designing, algae transformation by electroporation, selection on antibiotic plates, PCR amplification for GM confirmation, Southern hybridization to confirm gene integration, Western blotting to check protein expression, pigment quantification, and algae growth determination.
Databáze: Supplemental Index