| Autor: |
Akinpelu, Sharon, Ajayi, Abraham, Smith, Stella Ifeanyi, Adeleye, Adeyemi Isaac |
| Zdroj: |
Journal of Infection Prevention; July 2024, Vol. 25 Issue: 4 p126-133, 8p |
| Abstrakt: |
Background Multidrug resistant bacterial pathogens employ different mechanisms in evading the action of antibiotics. Multidrug resistance is wide spread among strains of Escherichia coliimplicated in several infections including urinary tract infections, gastrointestinal infections, meningitis and bacteraemia.Aim/Objective This study investigates the antibiotic resistance profile, efflux pump activity and biofilm formation ability of E. colistrains isolated from clinical samples.Methods A total of 32 E. colistrains isolated from clinical samples were characterized and subjected to antibiotic susceptibility testing using standard methods. Isolates were screened phenotypically for biofilm formation and efflux pump activity. While molecular detection of genes encoding curli fimbriae and efflux pump activity was done by PCR.Results All 32 (100%) E. coliisolates were resistant to ceftazidime, cefuroxime, cefixime, amoxicillin-clavulanate, ofloxacin and ciprofloxacin. While 30 (93.8%) were resistant to gentamicin, 27 (84.4%) were resistant to cefepime and the least resistance of 15.6% was to imipenem. Efflux pump encoding gene tolCwas detected in 13(40.6%) of the isolates, while 1(3.1%) harboured acrAgene. acrBgene was not detected in any of the isolates. Seven (21.9%) of the isolates were strong biofilm formers, while 5 (15.6%) and 20 (62.5%) were moderate and weak biofilm formers respectively. csgAgene was detected in all E. coliisolates.Discussion High antibiotic resistance of E. colistrains observed in this study is of public health significance. . It is therefore important to scale up efforts in regular monitoring of antibiotic resistance in both community and hospital settings. |
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