| Autor: |
Huo, Yuzhu, Cheng, Mengxue, Tang, Meiju, Zhang, Meng, Yang, Xiaofan, Zheng, Yating, Zhao, Tong, He, Peng, Yu, Jianing |
| Zdroj: |
Plant Communications; 20240101, Issue: Preprints |
| Abstrakt: |
Cotton is one of the most important textile fibers worldwide. As crucial agronomic traits, leaves play an essential role in the growth of cotton plants, disease resistance, fiber quality, and yield. Pentatricopeptide repeat (PPR) proteins are a large family of nuclear-encoded proteins involved in organellar or nuclear RNA metabolism. Using a virus-induced gene silencing (VIGS) assay, we found that cotton plants displayed variegated yellow leaf phenotypes with decreased chlorophyll content when silencing the expression of the PPR gene named GhCTSF1. GhCTSF1encodes a chloroplast-localized protein containing only two PPR motifs. Disruption of GhCTSF1substantially reduced the splicing efficiency of rpoC1intron 1 and ycf3intron 2. Loss of function of the GhCTSF1 ortholog EMB1417 causes splicing defects in rpoC1and ycf3-2, leading to impaired chloroplast structure and decreased photosynthetic rates in Arabidopsis. Moreover, we found that GhCTSF1interacted with two splicing factors, GhCRS2 and GhWTF1. Defects in GhCRS2 and GhWTF1 severely affect the intron splicing of rpoC1and ycf3-2 in cotton, leading to defects in chloroplast development and a reduction in photosynthesis. Our results suggest that GhCTSF1is specifically required for splicing rpoC1and ycf3-2 in cooperation with GhCRS2 and GhWTF1. |
| Databáze: |
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