| Abstrakt: |
Bacillus cereus sensu stricto(s.s.) is a well-known foodborne pathogen that produces a range of enterotoxins and is able to cause two different types of foodborne illnesses—the emetic and the diarrheal syndromes. In this study, 54 B. cereus s.s.strains isolated from foodstuff and foods involved in food poisoning outbreaks were characterized according to the presence of toxin-encoding genes, virulence-encoding genes, and panCtyping. Most isolates were assigned to panCgroups IV (61.1%) and III (25.9%), but members of groups II and V could also be found. Investigation of specific alleles revealed high numbers of isolates carrying toxin and other virulence genes including nheA(100%), nheB(100%), hblA(79.6%), hblC(79.6%), hblD(74.1%), cytK-2(61.1%), clo(100%), pc-plc(75.9%), sph(68.5%), pi-plc(66.6%), hlyIII(62.9%), and hlyII(24.1%). All isolates were negative for cesand cytK-1. In summary, we detected various enterotoxin and other virulence factor genes associated with diarrheal syndrome in strains analyzed, implicated or not with food poisoning.Furthermore, the most isolates analyzed belong to high-risk phylogenetic groups’ panCtypes III and IV. Our study provides a convenient molecular scheme for characterization of B. cereus s.s.strains responsible for food poisoning outbreaks in order to improve the monitoring and investigation and assess emerging clusters and diversity of strains. |
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