| Autor: |
AbuAlia, Khawla F N, Damm, Elena, Ullrich, Kristian K, Mukaj, Amisa, Parvanov, Emil, Forejt, Jiri, Odenthal-Hesse, Linda |
| Zdroj: |
Genetics; March 2024, Vol. 226 Issue: 3 |
| Abstrakt: |
PRDM9-mediated reproductive isolation was first described in the progeny of Mus musculus musculus(MUS) PWD/Ph and Mus musculus domesticus(DOM) C57BL/6J inbred strains. These male F1hybrids fail to complete chromosome synapsis and arrest meiosis at prophase I, due to incompatibilities between the Prdm9gene and hybrid sterility locus Hstx2. We identified 14 alleles of Prdm9in exon 12, encoding the DNA-binding domain of the PRDM9 protein in outcrossed wild mouse populations from Europe, Asia, and the Middle East, 8 of which are novel. The same allele was found in all mice bearing introgressed t-haplotypes encompassing Prdm9. We asked whether 7 novel Prdm9alleles in MUS populations and the t-haplotype allele in 1 MUS and 3 DOM populations induce Prdm9-mediated reproductive isolation. The results show that only combinations of the dom2allele of DOM origin and the MUS msc1allele ensure complete infertility of intersubspecific hybrids in outcrossed wild populations and inbred mouse strains examined so far. The results further indicate that MUS mice may share the erasure of PRDM9msc1binding motifs in populations with different Prdm9alleles, which implies that erased PRDM9 binding motifs may be uncoupled from their corresponding Prdm9alleles at the population level. Our data corroborate the model of Prdm9-mediatedhybrid sterility beyond inbred strains of mice and suggest that sterility alleles of Prdm9may be rare. |
| Databáze: |
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