| Autor: |
De Anda-Mora, Karla L., Tavares-Carreón, Faviola, Alvarez, Carlos, Barahona, Samantha, Becerril-García, Miguel A., Treviño-Rangel, Rogelio J., García-Contreras, Rodolfo, Andrade, Angel |
| Zdroj: |
Polish Journal of Microbiology; March 2024, Vol. 73 Issue: 1 p11-20, 10p |
| Abstrakt: |
Serratia marcescensis a global opportunistic pathogen. In vitrocytotoxicity of this bacterium is mainly related to metalloprotease serralysin (PrtS) activity. Proteolytic capability varies among the different isolates. Here, we characterized protease production and transcriptional regulators at 37°C of two S. marcescensisolates from bronchial expectorations, HU1848 and SmUNAM836. As a reference strain the insect pathogen S. marcescensDb10 was included. Zymography of supernatant cultures revealed a single (SmUNAM836) or double proteolytic zones (HU1848 and Db10). Mass spectrometry confirmed the identity of PrtS and the serralysin-like protease SlpB from supernatant samples. Elevated proteolytic activity and prtSexpression were evidenced in the HU1848 strain through azocasein degradation and qRT-PCR, respectively. Evaluation of transcriptional regulators revealed higher eepRexpression in HU1848, whereas cpxRand hexStranscriptional levels were similar between studied strains. Higher eepRexpression in HU1848 was further confirmed through an in vivotranscriptional assay. Moreover, two putative CpxR binding motifs were identified within the eepRregulatory region. EMSA validated the interaction of CpxR with both motifs. The evaluation of eepRtranscription in a cpxRdeletion strain indicated that CpxR negatively regulates eepR. Sequence conservation suggests that regulation of eepRby CpxR is common along S. marcescensspecies. Overall, our data incorporates CpxR to the complex regulatory mechanisms governing eepRexpression and associates the increased proteolytic activity of the HU1848 strain with higher eepRtranscription. Based on the global impact of EepR in secondary metabolites production, our work contributes to understanding virulence factors variances across S. marcescensisolates. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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