Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae

Autor: Boyer, Jeanne, Badis, Gwenaël, Fairhead, Cécile, Talla, Emmanuel, Hantraye, Florence, Fabre, Emmanuelle, Fischer, Gilles, Hennequin, Christophe, Koszul, Romain, Lafontaine, Ingrid, Ozier-Kalogeropoulos, Odile, Ricchetti, Miria, Richard, Guy-Franck, Thierry, Agnès, Dujon, Bernard
Zdroj: Genome Biology; August 2004, Vol. 5 Issue: 9 pR72-R72, 1p
Abstrakt: We have screened the genome of Saccharomyces cerevisiae for fragments that confer a growth-retardation phenotype when overexpressed in a multicopy plasmid with a tetracycline-regulatable (Tet-off) promoter. We selected 714 such fragments with a mean size of 700 base-pairs out of around 84,000 clones tested. These include 493 in-frame open reading frame fragments corresponding to 454 distinct genes (of which 91 are of unknown function), and 162 out-of-frame, antisense and intergenic genomic fragments, representing the largest collection of toxic inserts published so far in yeast.
Databáze: Supplemental Index
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