| Autor: |
Clements, Melanie, Simpson Ragdale, Holly, Garcia-Diaz, Claudia, Parrinello, Simona |
| Zdroj: |
STAR Protocols; March 2024, Vol. 5 Issue: 1 |
| Abstrakt: |
Disease-relevant in vivotumor models are essential tools for both discovery and translational research. Here, we describe a highly genetically tractable technique for generating immunocompetent somatic glioblastoma (GBM) mouse models using piggyBac transposition and CRISPR-Cas9-mediated gene editing in wild-type mice. We describe steps to deliver plasmids into subventricular zone endogenous neural stem cells by injection and electroporation, leading to the development of adult tumors that closely recapitulate the histopathological, molecular, and cellular features of human GBM. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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