| Autor: |
Wang, Haitao, Zhang, Yali, Dai, Dadong, Fu, Jinqiu, Sung Kim, Dae, Li, Shengchun, Zhang, Jiang, Wang, Yong, Zhang, Fengjuan |
| Zdroj: |
Journal of Economic Entomology; February 2024, Vol. 117 Issue: 1 p82-92, 11p |
| Abstrakt: |
Pseudomonas fluorescensgroup, such as Pseudomonas protegensand Pseudomonas chlororaphis, can be utilized as insect-killing agents. Most insecticidal Pseudomonasdescribed so far have high toxicity for insects of the order Lepidoptera. In this study, Pseudomonasstrain PcR3-3 was isolated from the willow root. It showed a high mortality for the coleopteran species Plagiodera versicolora(Coleoptera: Chrysomelidae), but not for the lepidopteran Helicoverpa armigera.Strain PcR3-3 displayed high colonization ability in the P. versicoloracompared with P. chlororaphisPCL1391, indicating that the insecticidal activities correlated with the colonization ability of Pseudomonasstrain in the host. Phylogenetic analysis of the genome revealed that PcR3-3 belonged to P. chlororaphissubsp. aureofaciens.Numerous insecticidal protein-encoding genes, typical biosynthetic gene clusters for some insecticidal metabolite and type VI secretion system, known to be involved in insect pathogenicity, were present in the P. chlororaphisPcR3-3 genome. However, the insecticidal toxin Fit-encoding gene which commonly presents in P. chlororaphis, was not found in the P. chlororaphisPcR3-3 genome. Furthermore, there are some divergent insecticidal genes between P. chlororaphisPcR3-3 and P. chlororaphisPCL1391. This finding implies that P. chlororaphisPcR3-3 is a promising biocontrol agent for pest management applications. The P. chlororaphis–P. versicoloraassociation can be used as a model system to study the interaction between Pseudomonasand coleopteran insects. |
| Databáze: |
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