| Abstrakt: |
The process of isolation and characterization entails examining soil samples from various conditions, followed by conducting biochemical and molecular tests to determine the profiles of biodegradable enzymes in chosen bacterial strains. The focus is mostly on enzymes responsible for breaking down resistant organic substances, highlighting the bacteria’s capacity to enhance soil health and promote the long-term stability of ecosystems. This study provides a thorough analysis of the isolation and characterization of a previously unidentified Bacillusstrain that exhibits exceptional dual enzymatic properties. The investigation explores this new Bacillus’scapacity for biodegradation, concentrating on its proteolytic and cellulolytic properties. After a variety of environmental samples were screened during the isolation procedure, a Bacillusstrain with a distinct enzyme repertoire was eventually discovered. Extensive biochemical and molecular investigations were carried out to clarify the enzymatic apparatus of this microbe, emphasizing its possible uses in biotechnological procedures. By breaking down cellulose substrates, the Bacillusstrain’s cellulolytic activity was investigated, demonstrating its ability to use cellulose as a source of carbon. The proteolytic activities were investigated concurrently, highlighting the strain’s potent protein hydrolyzing capacity. Additionally, the study looks at the possible synergistic effects of the two enzymatic activities, indicating intriguing uses in several industrial applications, such as the processing of proteins, waste management, and the manufacture of biofuel. The identification of important enzymes involved in the breakdown of proteins and cellulose provides important information for upcoming developments in biotechnology. This study adds to our knowledge of microbial variety and emphasizes the need to use new Bacillus strains with dual enzymatic capabilities for effective and sustainable biodegradation processes. The information provided here paves the way for additional research into and use of this Bacillusstrain in many biotechnological contexts. The highest enzyme activity was obtained at pH 7 with cellulases 2.055 U mg per min and proteases 211U ml per min, and the highest enzyme activity was obtained at temperature 37° with cellulases 2.440 U mg/ml. Maximum cellulase and protease enzyme activity was in the presence of sucrose around 2.88U/mg and 286U/ml, respectively, and providing the nitrogen supply in the form of yeast extract, it was cellulases 2.307 U/mg and proteases260U/ml. With the UV exposure and EMS treatment the maximum proteases and cellulases activities were achieved at 100 μl, 345 U/ml, and 3.840 U/mg/min, Similarly UV exposure for enhanced cellulases and proteases activities by 3868 U/mg/min and 357 U/ml. |
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