| Abstrakt: |
Human milk reduces the incidence of necrotizing enterocolitis (NEC). Prior studies have demonstrated that exogenous surfactant protein‐A (SP‐A) modulates intestinal inflammation, reduces NEC‐like pathology in SP‐A‐deficient (SPAKO) pups, and may contribute to breast milk's immunomodulatory potential. We hypothesize that SP‐A is present in milk and impacts inflammatory responses in the terminal ileum of neonatal mice. Human milk was collected at postpartum days 1–3 and 28. Mouse milk was collected at postpartum days 1–10. SP‐A was detected in milk through immunoprecipitation and western blot analysis. The impact of murine wild‐type (WT) milk on SPAKO pup ileum was evaluated in a model of intestinal inflammation via cross‐rearing experiments. Terminal ileum was evaluated for inflammatory cytokine and toll‐like receptor 4 (TLR4) mRNA expression via quantitative real‐time RT‐PCR. SP‐A was detected in human milk and wild type (WT) mouse milk, but not in SPAKO mouse milk. Expression of TLR4, interleukin (IL)‐1β, IL‐6, and tumor necrosis factor (TNF)‐α was decreased in SPAKO pups reared with WT dams compared to SPAKO pups reared with SPAKO dams, with a peak effect at day of life 14. When inflammation was induced using a lipopolysaccharide‐induced model of inflammation, expression of TLR4, IL‐1β, IL‐6, CXCL‐1, and TNF‐α was significantly lower in SPAKO pups reared with WT dams compared to SPAKO pups reared with SPAKO dams. SP‐A is present in human and murine milk and plays a role in lowering inflammation in murine pup terminal ileum. Both baseline inflammation and induced inflammatory responses are reduced via exposure to SP‐A in milk with the effect amplified in inflammatory conditions. |
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