| Abstrakt: |
This study was conducted to determine the antimicrobial resistance profiles of Escherichia coliisolated from food samples received at the National Food Safety Agency in Burkina Faso. A total of 155 isolates from dairy foods (29), fish-based dishes (7), mango juices (4), lettuces (35), RTE salads (21), sandwiches (32), and sesames (27) were included for testing with the Kirby-Bauer disk diffusion method. PCR targeting ampicillin (blaTEM, blaSHV, temA, and temB), tetracyclines [tet(A)and tet(B)], sulfamethoxazole (sul1and sul2), aminoglycosides (StrAand aadA) and quinolones (GyrA) resistance genes were performed to elucidate the genotypic resistance mechanism. Of the 155 isolates, 105 (67.7%) were resistant to at least one antimicrobial agent. Resistances to tetracycline (33.5%), ampicillin (32.9%), cefoxitin (18.7%), gentamycin (15.5%), amoxicillin-clavulanate acid (15.5%), nalidixic acid (12.9%), chloramphenicol (11.6%), trimethoprim-sulfamethoxazole (11.6%), and ciprofloxacin (8.4%) were observed. Multidrug resistance was recorded in 26.5% of the isolates. Antimicrobial resistance genes including blaTEM(19/51, 37.3%), blaSHV(19/51, 37.3%), temB(17/51, 33.3%), tet(A)(24/52, 46.2%), tet(B)(9/52, 17.3%), sul1(8/18, 44.4%), sul2(4/18, 22.2%), aadA(11/24, 45.83%) and GyrA(31/36, 86.1%) were detected. All E. coliisolates resistant to at least 2 antibiotics were positive for the class 1 integron gene (intI1). These findings raise concerns about food safety and public health and demonstrate the need for strict government control and continuous monitoring. |
Full text from SpringerLink