| Autor: |
Liu, Jiyuan, Tian, Zhen, Li, Ruichi, Ni, Shujun, Sun, Hong, Yin, Fei, Li, Zhenyu, Zhang, Yalin, Li, Yifan |
| Zdroj: |
Journal of Agricultural and Food Chemistry; February 2024, Vol. 72 Issue: 5 p2560-2572, 13p |
| Abstrakt: |
The overexpression of insect detoxification enzymes is a typical adaptive evolutionary strategy for insects to cope with insecticide pressure. In this study, we identified a glutathione S-transferase (GST) gene, PxGSTs1, that exhibited pronounced expression in the field-resistant population of Plutella xylostella. By using RNAi (RNA interference), the transgenic fly models, and quantitative real-time polymerase chain reaction (RT-qPCR) methods, we confirmed that the augmented expression of PxGSTs1mediates the resistance of P. xylostellato various types of insecticides, including chlorantraniliprole, novaluron, λ-cyhalothrin, and abamectin. PxGSTs1was found to bolster insecticide resistance in two ways: direct detoxification and enhancing antioxidative defenses. In addition, our findings demonstrated that pxy-miR-8528a exerts a pivotal influence on forming insecticide resistance in P. xylostellaby downregulating PxGSTs1expression. In summary, we elucidated the multifaceted molecular and biochemical underpinnings of PxGSTs1-driven insecticide resistance in P. xylostella. Our results provide a new perspective for understanding the insecticide resistance mechanism of P. xylostella. |
| Databáze: |
Supplemental Index |
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