| Autor: |
Deng, Yinai, Yang, Peng, Zhang, Qianle, Wu, Qingwen, Feng, Lingfang, Shi, Wenjing, Peng, Qian, Ding, Li, Tan, Xukai, Zhan, Ruoting, Ma, Dongming |
| Zdroj: |
Cell Reports; 20240101, Issue: Preprints |
| Abstrakt: |
Flavonoids are a class of secondary metabolites widely distributed in plants. Regiospecific modification by methylation and glycosylation determine flavonoid diversity. A rare flavone glycoside, diosmin (luteolin-4'-methoxyl-7-O-glucosyl-rhamnoside) occurs in Chrysanthemum indicum. How Chrysanthemumplants evolve new biosynthetic capacities remains elusive. Herein, we assembled a 3.11 Gb high-quality C. indicumgenome with a contig N50 value of 4.39 Mb and annotated 50,606 protein-coding genes. One (CiCOMT10) of tandemly repeated O-methyltransferase genes undergoes neofunctionalization, preferentially transferring the methyl group to the 4'-hydroxyl group of luteolin with ortho-substituents to form diosmetin. In addition, CiUGT11 (UGT88B3) specifically glucosylates 7-OH group of diosmetin. Next, we construct a one-pot cascade biocatalyst system by combining CiCOMT10, CiUGT11, and our previously identified rhamnosyltransferase, effectively producing diosmin with over 80% conversion from luteolin. This study clarifies the role of transferases in flavonoid diversity and provides the important gene elements essential for producing rare flavone. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
