| Autor: |
Souza, João Gabriel Silva, Bertolini, Martinna, Thompson, Angela, Mansfield, Jillian M, Grassmann, André Alex, Maas, Kendra, Caimano, Melissa J, Barao, Valentim Adelino Ricardo, Vickerman, M Margaret, Dongari-Bagtzoglou, Anna |
| Zdroj: |
The ISME Journal; May 2020, Vol. 14 Issue: 5 p1207-1222, 16p |
| Abstrakt: |
Streptococcal glucosyltransferases (Gtf) synthesize a-glucan exopolymers which contribute to biofilm matrix. Streptococcus oralisinteracts with the opportunistic pathogen Candida albicansto form hypervirulent biofilms. S. oralis34 has a single gtfgene (gtfR). However, the role of gtfRin single and mixed species biofilms with C. albicanshas never been examined. A gtfRdeletion mutant, purified GtfR, and recombinant GtfR glucan-binding domain were tested in single and mixed biofilms on different substrata in vitro. A mouse oral infection model was also used. We found that in single species biofilms growing with sucrose on abiotic surfaces S. oralis gtfRincreased biofilm matrix, but not bacterial biomass. In biofilms with C. albicans, S. oralisencoding gtfRshowed increased bacterial biomass on all surfaces. C. albicanshad a positive effect on a-glucan synthesis, and a-glucans increased C. albicansaccretion on abiotic surfaces. In single and mixed infection of mice receiving sucrose S. oralis gtfRenhanced mucosal burdens. However, sucrose had a negative impact on C. albicansburdens and reduced S. oralisburdens in co-infected mice. Our data provide new insights on the GtfR-mediated interactions between the two organisms and the influence of biofilm substratum and the mucosal environment on these interactions. |
| Databáze: |
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