| Autor: |
Lima, Alan Kelbis Oliveira, Silveira, Ariane Pandolfo, Silva, Renata Carvalho, Machado, Yasmin Alves Aires, de Araújo, Alyne Rodrigues, de Mendonça Araujo, Sansara Sanny, Vieira, Italo Rennan Sousa, Araújo, Joabe Lima, dos Santos, Lucas Carvalho, da França Rodrigues, Klinger Antônio, da Silva, Sebastião William, de Aquino Ribeiro, José Antônio, Rodrigues, Clenilson Martins, Garcia, Mônica Pereira |
| Zdroj: |
Biomass Conversion and Biorefinery; 20240101, Issue: Preprints p1-17, 17p |
| Abstrakt: |
The optimization techniques are fundamental to guarantee the stability of any preparation process and the quality of the nanostructures synthesized under ideal circumstances. In this sense, this study evaluates the potential of guarana leaf extract (Paullinia cupanaKunth—Sapindaceae) for the green synthesis of silver nanoparticles (AgNPs) using routes that employ different equipment/energy sources (autoclave, LED, microwave, sunlight, ultrasound, and water bath). The aqueous extract was characterized by high-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS/MS). The AgNPs were analyzed by UV/Vis spectroscopy, dynamic light scattering (DLS), zeta potential (ZP), Fourier transform infrared (FTIR), transmission electron microscopy (TEM) and their antibacterial, leishmanicidal and cytotoxic effects were evaluated in vitro. The results show the presence of phenolic acids, alkaloids, and flavonoids in the leaf extract, which was supported by the identification of their functional groups in the FTIR spectra. The AgNPs showed maximum absorption between 420 and 440 nm (UV/Vis), with a diameter below 100 nm (DLS) and 60 nm (TEM), spherical morphology and a surface charge above − 30 mV (ZP). The antibacterial test showed pronounced inhibition in the growth of Escherichia coli, Pseudomonas aeruginosaand Staphylococcus aureusbacteria. The leishmanicidal activity of AgNPs on Leishmania (Leishmania) amazonensiswas more pronounced than that of the drug miltefosine while low cytotoxicity was reported on RAW 264.7 macrophages. With this, this study reports the possibility of using alternative routes for the sustainable synthesis of AgNPs from Paullinia cupanaextract with considerable applications against pathogenic bacteria and opportunistic parasites. |
| Databáze: |
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