FOXP1 and KLF2 reciprocally regulate checkpoints of stem-like to effector transition in CAR T cells

Autor: Zhu, Ziang, Lou, Guohua, Teng, Xiao-Lu, Wang, Haixia, Luo, Ying, Shi, Wangke, Yihunie, Kiddist, Hao, Shumeng, DeGolier, Kole, Liao, Chengheng, Huang, Huocong, Zhang, Qing, Fry, Terry, Wang, Tao, Yao, Chen, Wu, Tuoqi
Zdroj: Nature Immunology; 20230101, Issue: Preprints p1-12, 12p
Abstrakt: In cancer and infections, self-renewing stem-like CD8+T cells mediate the response of immunotherapies and replenish terminally exhausted T cells and effector-like T cells. However, the programs governing the lineage choice in chimeric antigen receptor (CAR) T cells are unclear. Here, by simultaneously profiling single-cell chromatin accessibility and transcriptome in the same CAR T cells, we identified heterogeneous chromatin states within CD8+T cell subsets that foreshadowed transcriptional changes and were primed for regulation by distinct transcription factors. Transcription factors that controlled each CD8+T cell subset were regulated by high numbers of enhancers and positioned as hubs of gene networks. FOXP1, a hub in the stem-like network, promoted expansion and stemness of CAR T cells and limited excessive effector differentiation. In the effector network, KLF2 enhanced effector CD8+T cell differentiation and prevented terminal exhaustion. Thus, we identified gene networks and hub transcription factors that controlled the differentiation of stem-like CD8+CAR T cells into effector or exhausted CD8+CAR T cells.
Databáze: Supplemental Index
Externí odkaz: