| Autor: |
Song, Ya-Fei, Chu, Meng-Jie, Huang, Fei, Liu, Yang, Lu, Hui-Hong, Li, Si-Ang, Wang, Shu-Yan, Song, Ya-Fei, Chu, Meng-Jie, Huang, Fei, Liu, Yang, Lu, Hui-Hong, Li, Si-Ang, Wang, Shu-Yan |
| Zdroj: |
Parasite - Journal de la Société Française de Parasitologie; January 2023, Vol. 30 Issue: 1 |
| Abstrakt: |
Giardia duodenalisis a common intestinal protozoan that can cause diarrhea and intestinal disease in animals and in humans. However, the prevalence and assemblages of G. duodenalisin pigs from Guangxi Zhuang Autonomous Region have not been reported. In this study, a total of 724 fecal samples (201 from nursery pigs, 183 from piglets, 175 from breeding pigs, and 165 from fattening pigs) were obtained in four areas of the region (Nanning, Yulin, Hezhou, and Guigang). The gene of the small subunit ribosomal RNA (SSU rRNA) of G. duodenaliswas amplified by nested PCR. The results show that the prevalence of G. duodenalisin pigs was 3.59% (26/724), of which 14 samples belonged to assemblage A (53.85%) and 12 samples belonged to assemblage E (46.15%). The infection rates of G. duodenalisin Hezhou, Yulin, Nanning, and Guigang were 0%, 0.7%, 10.8% and 1.1%, respectively (χ2= 45.616, p< 0.01); whereas 5.1% of breeding pigs, 6.0% of piglets, 2.4% of fattening pigs, and 1.0% of nursery pigs were infected with G. duodenalis(χ2= 8.874, p< 0.05).The SSU rRNA-positive samples were amplified by PCR based on the β-giardin (bg), glutamate dehydrogenase (gdh), and triphosphate isomerase (tpi) genes. Ten, eight and seven positive samples were detected, respectively. Based on phylogenetic analysis of the three genetic loci sequences, a multilocus genotyping A1 was found. The findings of this study provide basic data for the development of prevention and control of G. duodenalisinfections in pigs and humans in the Guangxi Zhuang Autonomous Region. |
| Databáze: |
Supplemental Index |
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