Traditional marketed meats as a reservoir of multidrug-resistant Escherichia coli

Autor: Guibert, Fernando, Espinoza, Kathya, Taboada-Blanco, Clara, Alonso, Carla A., Oporto, Rosario, Castillo, Angie K., Rojo-Bezares, Beatriz, López, María, Sáenz, Yolanda, Pons, Maria J., Ruiz, Joaquim
Zdroj: International Microbiology; 20230101, Issue: Preprints p1-17, 17p
Abstrakt: This study aimed to analyze Escherichia colifrom marketed meat samples in Peru. Sixty-six E. coliisolates were recovered from 21 meat samples (14 chicken, 7 beef), and antimicrobial resistance levels and the presence of mechanisms of antibiotic resistance, as well as clonal relationships and phylogeny of colistin-resistant isolates, were established. High levels of antimicrobial resistance were detected, with 93.9% of isolates being multi-drug resistant (MDR) and 76.2% of samples possessing colistin-resistant E. coli; of these, 6 samples from 6 chicken samples presenting mcr-1-producer E. coli. Colistin-resistant isolates were classified into 22 clonal groups, while phylogroup A (15 isolates) was the most common. Extended-spectrum β-lactamase- and pAmpC-producing E. coliwere found in 18 and 8 samples respectively, with blaCTX-M-55(28 isolates; 16 samples) and blaCIT(8 isolates; 7 samples) being the most common of each type. Additionally, blaCTX-M-15, blaCTX-M-65, blaSHV-27, blaOXA-5/10-like, blaDHA, blaEBCand narrow-spectrum blaTEMwere detected. In addition, 5 blaCTX-Mremained unidentified, and no sought ESBL-encoding gene was detected in other 6 ESBL-producer isolates. The tetA, tetEand tetXgenes were found in tigecycline-resistant isolates. This study highlights the presence of MDR E. coliin Peruvian food-chain. The high relevance of CTX-M-55, the dissemination through the food-chain of pAmpC, as well as the high frequency of unrelated colistin-resistant isolates is reported.
Databáze: Supplemental Index
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