| Abstrakt: |
Backgrounds: Tissue inhibitors of metalloproteinases (TIMPs) are endogenous inhibitors of active MMPs. TIMP-2 and TIMP-3 are cell cycle regulatory genes found normally in breast epithelium. Changes in the expression of TIMP-2 and TIMP-3 genes in breast tumors and surrounding tissue are related to the disruption of cell cycle regulation and tumor cell proliferation. This study aimed to investigate the association between the TIMP-2 rs8179090 and TIMP-3 rs9619311 gene polymorphisms with breast cancer risk in an Iranian population. Methods: In a case–control design, 300 women subjects including 150 breast cancer samples and 150 healthy samples were included. Polymorphism analysis of TIMP-2 and TIMP-3 genes was done by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) technique, and genotypes were confirmed by direct sequencing of PCR products. Results: The results showed that rs8179090 polymorphism in heterozygous, dominant, and allelic models is associated with an increased risk of breast cancer. Also, regarding rs9619311 polymorphism, this variant was associated with an increased risk of breast cancer in homozygous and allelic models. Conclusions: Both studied genetic polymorphisms could be considered genetic risk factors for breast cancer in an Iranian women population. As a result, these gene polymorphisms can be considered potential biomarkers for breast cancer screening. |
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