| Abstrakt: |
Gut microbiomes play a significant role in the growth, metabolism, and health of fish. However, we know very little about interactive effects of variations of dietary composition and temperature on rainbow trout gut microbiota. Exactly 288 rainbow trout weighing 45.6g ± 0.05 (average ± SD) were fed four isocaloric, isolipidic, and isonitrogenous diets comprised 40% crude protein and 20% crude lipid and formulated as 100 % animal-based protein (AP) and blend of 50 fish oil (FO)/50 camelina oil (CO), 100 % AP and100 % CO, 100 % plant-based protein (PP) and blend of 50FO/50CO or 100 % PP and 100 % CO in 14 or 18°C for 150 days. Gut content was analyzed using 16S rRNA gene and shotgun sequencing. The most abundant phyla identified regardless of diet were Tenericutes, Firmicutes, Proteobacteria, Spirochaetes, Bacteroidetes, and Actinobacteriawhile Aeromonadaceaeand Enterobacteriaceaewere dominant families in 18°C. Moreover, gut microbes were dominated by genes relating to amino acid, carbohydrate, fat, and energy metabolisms and influenced by temperature. The shared functional profiles for all the diets suggest that plant protein sources in combination with CO could be as good as the fish meal with 50/50 FO and CO in rainbow trout farming. |
