Autor: |
Al-khaldi, Sufian F., Mossoba, Magdi M., Ismail, Ashraf A., Fry, Fred S. |
Zdroj: |
Foodborne Pathogens & Disease; Fall 2004, Vol. 1 Issue: 3 p172-177, 6p |
Abstrakt: |
A microarray method for the deposition of bacteria onto an agar slide was developed to accelerate the formation of microcolonies. Representative microarrays each consisting of 40 micro-spots of five replicates of eight foodborne bacteria (Yersinia enterocolitica, Staphylococcus aureus, Salmonella typhimurium, Listeria monocytogenes, Enterobacter cloacae, Citrobacter freundii, Klebsiella pneumoniae, and Escherichia coli) were printed on a Brain Heart Infusion (BHI) agar slide using a contact micro-spotting robotic system. Within 3 h, sufficient bacterial cells were obtained to allow accurate identification of the microorganism by infrared spectroscopy. This approach allows a "complete-in-a-single-day" analysis of a large array of samples. |
Databáze: |
Supplemental Index |
Externí odkaz: |
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