| Autor: |
Codling, Caroline E., Jones, Brian V., Mahenthiralingam, Eshwar, Russell, A. Denver, Maillard, Jean-Yves |
| Zdroj: |
Journal of Antimicrobial Chemotherapy (JAC); August 2004, Vol. 54 Issue: 2 p370-370, 1p |
| Abstrakt: |
<it>Objectives</it>: Polyquaternium-1 (PQ-1) is a biocide used commercially in a contact lens disinfecting solution, ‘Opti-Free Express (Alcon) Multi-Purpose Disinfecting Solution’. The genetic basis for resistance of <it>Serratia marcescens</it> to PQ-1 was investigated using a random transposon-based mutagenesis approach. <it>Methods</it>: <it>S. marcescens</it> was subjected to random transposon mutagenesis using a mini-Tn<it>5</it> Km2 transposon. Mutants with increased susceptibility to PQ-1 were selected and the disrupted genes were identified. Antibiotic susceptibility profiles were also determined for all of the mutants. <it>Results</it>: A wide range of genes were found to be disrupted in the mutants. The most common were genes associated with the cell membranes, or involved in biosynthesis and metabolism. <it>Conclusions</it>: This study shows that random transposon mutagenesis is an effective tool for the elucidation of mechanisms of action and resistance to biocides. The results support our previous findings that PQ-1 is active against the cytoplasmic membrane of <it>S. marcescens</it>. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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