Comparison of disc-diffusion and disc-volatilization assays for determining the antimicrobial activity of Thymus vulgarisL. essential oil

Autor: Ignatiuk, Katarzyna, Dzikon, Ewelina, Hagdej, Brygida, Slotwinska, Weronika, Malm, Maria, Ossowski, Mateusz, Kasela, Martyna
Zdroj: Current Issues in Pharmacy and Medical Sciences; September 2023, Vol. 36 Issue: 3 p158-162, 5p
Abstrakt: Essential oils (EOs), being mixtures of various natural compounds, constitute an attractive source of novel antimicrobial agents. Along with multiple studies concerning their rich biological activity, there are different methods allowing to determine both antimicrobial activity in direct contact, as well as through the activity of vapours. Thyme constitutes one of the most common aromatic herbs used for pharmaceutical, cosmetic and culinary purposes. The aim of the study was to compare the antibacterial activity of liquid and vapour phases of Thymus vulgarisL. essential oil (TEO) against most common pathogenic Gram-positive (Staphylococcus aureusATCC 25913, Enterococcus faecalisATCC 29212, Bacillus cereusATCC 10876) and Gram-negative (Escherichia coliATCC 25922, Salmonella typhimuriumATCC 14028, Pseudomonas aeruginosaATCC 27853) microorganisms. To determine TEO antibacterial properties, two methods were used: disc-diffusion assay so as to test activity in direct contact; disc-volatilization assay so as to determine activity in gaseous contact.
Databáze: Supplemental Index