| Autor: |
Li, Peikun, Wang, Yanchao, Luo, Xiuyan, Geng, Zixin, Jia, Jiahui, Wang, Ruirui, Cui, Jiandong, Bian, Hongjie, Jia, Shiru |
| Zdroj: |
Particuology; 20230101, Issue: Preprints |
| Abstrakt: |
Herein, a new nanozyme/natural enzyme hybrid biosensor was established for ultrasensitive L-glutamic acid (L-Glu) detection. The Fe3O4nanoparticles with peroxidase-like activity and stability was used as a nanozyme and carrier for immobilizing L-Glutamate oxidase (GLOD) through Schiffff base reaction to construct a chem-enzyme cascade detector. The resultant Fe3O4-GOLD exhibited a wide linear range (10−500 μM) and a low detection limit of 6.03 μM for L-Glu detection. Furthermore, the Fe3O4-GOLD exhibited excellent pH stability, thermal stability, reusability and storage stability. After repeated nine cycles, Fe3O4-GOLD still retained 70% of its initial activity. Meanwhile, Fe3O4-GOLD maintained 50% of its initial activity after storage for 20 days, while free GLOD only retained 20% of its initial activity. This strategy of integrating biomimetic Fe3O4and natural enzymes for cascade catalysis makes it possible to design an efficient and stable chemo-enzyme composite catalysts, which are promising for applications in biosensing and biomimetic catalysis. |
| Databáze: |
Supplemental Index |
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