Enzymatic synthesis of β-d-fructofuranosyl α-d-glucopyranosyl-(1→2)-α-d-glucopyranoside using Escherichia coliglycoside phosphorylase YcjT

Autor: Isono, Naoto, Yagura, Shuichiro, Yamanaka, Kanon, Masuda, Yukino, Mukai, Kazuki, Katsuzaki, Hirotaka
Zdroj: Bioscience, Biotechnology, and Biochemistry; October 2023, Vol. 87 Issue: 10 p1249-1253, 5p
Abstrakt: YcjT is a kojibiose phosphorylase found in Escherichia coli. We found that sucrose was a good acceptor of YcjT in reverse phosphorolysis using β-d-glucose 1-phosphate as a donor. The product was identified as β-d-fructofuranosyl α-d-glucopyranosyl-(1→2)-α-d-glucopyranoside. This sugar was also synthesized from sucrose and maltose using YcjT and maltose phosphorylase and promoted the growth of the probiotic Bifidobacterium breve.
Databáze: Supplemental Index