Dissemination and characterization of Stenotrophomonas maltophiliaisolates from Dairy Cows in Northeast China

Autor: Shao, Lanjun, Liu, Xiaojing, Liu, Yi, Shen, Jie, Liu, Ruishan, Chen, Pingnan
Zdroj: Polish Journal of Microbiology; September 2023, Vol. 72 Issue: 3 p319-323, 5p
Abstrakt: This work investigated the genetic relationship among Stenotrophomonas maltophiliastrains in fecal samples from dairy cows in northeast China and identified the dominant β-lactamase genotype. One hundred and six samples were collected from two randomly selected cow farms in northeast China, and the isolates were identified with MALDI-TOF/MS. Whole-genome sequencing was conducted using Illumina HiSeq 4000-PE150 platform (Illumina, Inc., USA). The antimicrobial resistance genes were detected using CGE services. The phylogenetic analysis of S. maltophiliastrains was performed by Roary and MEGA X. In total, 24 S. maltophiliaisolates were isolated. The results of resistome analysis showed all S. maltophiliastrains carrying blaL1gene, which was the only β-lactamase genotype. In addition, the aminoglycoside resistance genes aac(6′)-Izand aph(3′)-IIcwere found. The phylogenetic tree indicated the clonal diversity of S. maltophiliain these two regions and the clonal relatedness of the strains from these regions. This study first investigated the dissemination and characterization of S. maltophiliaisolates from dairy cows in northeast China and provided evidence of the potential transmission between two provinces. Furthermore, it indicated blaL1was the most prevalent genotype of β-lactamase in these regions.
Databáze: Supplemental Index