Modified protocol for culturing DrosophilaS2 R+ cells and adult plasmatocytes to study actin cytoskeleton dynamics

Autor: Weaver, Ceileigh M., Makdissi, Stephanie, Di Cara, Francesca
Zdroj: STAR Protocols; September 2022, Vol. 3 Issue: 3
Abstrakt: Here, we describe a protocol to culture DrosophilaS2R+ cells and to extract plasmatocytes from adult flies. The modified seeding approach detailed here, in combination with coating of coverslips with concanvalin A, enables enhanced adhesion and spreading of cells. We describe the steps for confocal microscopy and a detailed quantification pipeline to evaluate changes in cortical actin cytoskeleton dynamics. The protocol can be applied to a variety of genetic or chemical perturbations.
Databáze: Supplemental Index