| Autor: |
da Cunha, Kamila Furtado, Oliveira Garcia, Marcelle, Allend, Suzane Olachea, de Albernaz, Déborah Farias Trota, Panagio, Luciano Aparecido, Neto, Amilton Clair Pinto Seixas, Larré Oliveira, Thaís, Hartwig, Daiane Drawanz |
| Zdroj: |
Brazilian Journal of Microbiology; December 2023, Vol. 54 Issue: 4 p2641-2650, 10p |
| Abstrakt: |
Multidrug-resistant (MDR) bacteria are one problem in health since the therapeutic alternative are reduced. For this, the application of nanotechnology through functionalized nanoparticles, like a biogenic silver nanoparticle (Bio-AgNP), obtained by biological synthesis, emerges as a possible alternative against the MDR bacteria. This study aimed to evaluate the antibacterial and antibiofilm activity of Bio-AgNP obtained for biological synthesis by Fusarium oxysporumstrain 551 against methicillin-resistant Staphylococcus aureus(MRSA) and MDR coagulase-negative Staphylococcus(CoNS) isolates. Bio-AgNP has activity against S. aureusATCC 25904, Staphylococcus epidermidisATCC 35984, and MDR isolates, with minimal inhibitory concentration (MIC) ranging from 3.75 to 15 μg.mL-1and minimal bactericidal concentration (MBC) from 7.5 to 30 μg.mL-1. In the membrane leakage assay, it was observed that all concentrations tested led to proteins release from the cellular content dose-dependently, where the highest concentrations led to higher protein in the supernatant. The 2×MIC of Bio-AgNP killed ATCC 35984 after 6h of treatment, and ATCC 25904 and S. aureus(SA3) strains after 24h of treatment. The 4×MIC was bactericidal in 6h of treatment for all strains in the study. The biofilm of MDR isolates was inhibited in 80.94 to 100% and eradicated in 60 to 94%. The confocal laser scanning microscopy (CLSM) analysis demonstrated similar results to the antibiofilm assays. The Bio-AgNP has antibacterial and antibiofilm activity and can be a promising therapeutic alternative against MDR bacteria. |
| Databáze: |
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