| Abstrakt: |
Biological Soil Disinfestation (BSD) is used to control soil-borne phytopathogens like Ralstonia solanacearumby introducing fresh organic matter and covering the soil with thick, opaque plastic sheets to induce soil anaerobiosis. In this study, BSD was implemented for the first time in Egypt to manage R. solanacearumin five naturally-infested fields. To improve BSD’s efficiency, biocontrol agents, including Pseudomonas putida(PD3142), P. fluorescens(PD3339), Acinetobacter baumannii(PD3138), and Stenotrophomonas maltophilia(PD4560), or plant-animal compost were applied after removing the plastic. The pathogen was eliminated when the soil oxygen concentration was less than or equal to 1%. However, this method resulted in a decrease in potato production. Application of P. putidaand P. fluorescensafter BSD led to a significant increase in potato production. Furthermore, BSD led to a significant decrease in fungal biodiversity, measured by the Shannon index (H’). PCR-DGGE analysis showed that P. putidaand S. maltophiliareduced bacterial biodiversity as indicated by species richness (S) and H’. Next generation sequencing assays showed Moraxellaceaeand Pseudomonadaceaedecrease in their abundance after BSD treatment, while Bacillaceaewere less affected . Moreover, applying of compost following BSD was associated with an increase in beneficial bacterial genera such as Luteolibacter, Dyadobacter, Sphingobium, Flavobacterium, and Pseudomonas. |
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