| Autor: |
Tong, Alina Y., Caudill, Emma E., Jones, Alexis R., F. M. Passalacqua, Luiz, Abdelsayed, Michael M. |
| Zdroj: |
Biochemistry; October 2023, Vol. 62 Issue: 20 p2902-2907, 6p |
| Abstrakt: |
RNA thermometers are noncoding RNA structures located in the 5′ untranslated regions (UTRs) of genes that regulate gene expression through temperature-dependent conformational changes. The fourU class of RNA thermometers contains a specific motif in which four consecutive uracil nucleotides are predicted to base pair with the Shine-Dalgarno (SD) sequence in a stem. We employed a bioinformatic search to discover a fourU RNA thermometer in the 5′-UTR of the blyAgene of the Bacillus subtilisphage SPβc2, a bacteriophage that infects B. subtilis168. blyAencodes an autolysin enzyme, N-acetylmuramoyl-l-alanine amidase, which is involved in the lytic life cycle of the SPβ prophage. We have biochemically validated the predicted RNA thermometer in the 5′-UTR of the blyAgene. Our study suggests that RNA thermometers may play an underappreciated yet critical role in the lytic life cycle of bacteriophages. |
| Databáze: |
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