| Autor: |
McDonald, Samuel C., Buck, James W., Li, Zenglu |
| Zdroj: |
Crop Science; September 2023, Vol. 63 Issue: 5 p2740-2749, 10p |
| Abstrakt: |
The fungal disease frogeye leaf spot (FLS) can reduce yield up to 30% in susceptible soybean [Glycine max(L) Merr] cultivars. The Rcs2locus in the cultivar Kent provides resistance to all the named Cercospora sojinaraces currently found in the United States; however, this locus for resistance to FLS has not been molecularly mapped yet. F2:3and recombinant inbred line (RIL) populations derived from a cross of Forrest × Kent were phenotyped in greenhouse assays with isolate S23 of C. sojina, and reactions were determined using a visual estimate of disease severity. A bulked segregant analysis and linkage mapping with Kompetitive allele‐specific PCR (KASP) markers in the F2:3population located the Rcs2locus on chromosome 11. The locus was mapped in the RIL population derived from the same pedigree and refined to a 336 kb interval by evaluating recombination break points in the RILs. This region contains 31 gene models, and Kent has polymorphisms in 11 of these genes and/or promoter regions of the Williams82.a2.v1 genome. The Rcs2locus and linked markers reported will be useful in marker‐assisted selection for FLS resistance. This research will also guide future efforts to understand the landscape of FLS resistance in United States soybean germplasm and to discover the causal genes for Rcs2resistance. Genetic mapping placed the Rcs2locus for resistance to frogeye leaf spot disease in soybean on chromosome 11.Fine mapping and sequence analysis narrowed the locus to 336 kb and 11 candidate genes.Markers developed to fine‐map Rcs2can be useful for the selection of resistance in breeding. |
| Databáze: |
Supplemental Index |
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