| Autor: |
Fägerstam, Lars G., Lizana, Jorge, Axiö-Fredriksson, Ulla-Britt, Wahlström, Lennart |
| Zdroj: |
Journal of Chromatography A; August 1983, Vol. 266 Issue: 1 p523-532, 10p |
| Abstrakt: |
A new chromatofocusing medium, MonoP™, was used for fast (60 min or less) separations of human serum proteins. Separations in the broad pH interval 6.0–3.8 were analysed by fused rocket immunoelectrophoresis to identify a number of proteins, and by gradient gel electrophoresis to determine the molecular weight distribution of the eluted material. To illustrate further the high resolving power of chromatofocusing, narrow pH intervals of about 0.5 pH units were used to study the microheterogeneity of α1-antitrypsin and Gc-globulin. Due to its high resolving power and preparative capacity, chromatofocusing is attractive as the first dimension in two-dimensional techniques for the resolution of complex protein mixtures. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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