| Autor: |
Power, S.D., Lochrie, M.A., Poyton, R.O. |
| Zdroj: |
Journal of Chromatography A; August 1983, Vol. 266 Issue: 1 p585-598, 14p |
| Abstrakt: |
Reversed-phase chromatography of the subunits of an oligomeric membrane protein such as yeast cytochrome coxidase requires additional sample handling techniques which are not necessary for soluble proteins. This paper considers these and discusses (1) methods for the removal of ballast material by preliminary batchwise extraction with solvent mixtures similar to those used for reversed-phase elution; (2) the chromatographic heterogeneity induced by partial cysteine oxidation; (3) the removal of tightly bound proteins from the stationary phase; and (4) the generation of an elution system with continuously variable selectivity based on acetonitrile-l-propanol ratios (0.05 % triethylamine, 0.05 % trifluoroacetic acid). |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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