| Abstrakt: |
ABSTRACTIntroductionThe combination of Virtual Screening (VS) techniques with in vivoscreening in the zebrafish model is currently being used in tandem for drug development in a faster and more efficient way.Areas coveredWe review the different virtual screening techniques, the use of zebrafish as a vertebrate model for drug discovery and the synergy that exists between them.Expert opinionWe highlight the advantages of combining virtual and zebrafish larvae screening for drug discovery. On the one hand, VS is a faster and cheaper tool for searching active compounds and possible candidates for therapy than in vivoscreening when processing large compound libraries. On the other hand, zebrafish larvae form a vertebrate model that allows in vivoscreening of large amounts of the compounds. Importantly, physiology and chemical response are mostly conserved between zebrafish and mammals. The availability of the transgenic and mutant zebrafish lines allows an analysis of a specific phenotype upon treatment, along with toxicity, off-target effect, side effects, and dosage. The advantages of VS, in vivowhole animal approach screening, and the screening combinations are also reviewed. |
