| Abstrakt: |
The emergence of drug resistant Mycobacterium tuberculosisstrains increases the burden on the treatment of tuberculosis. In this study, through in-silicotranscriptome analysis of drug-treated M. tuberculosissamples, novel drug targets for the treatment of drug resistance in tuberculosis were identified. Gene expression datasets of tuberculosis patients samples treated with different antibiotics (Isoniazid, Rifampicin, Pyrazinamide, Bedaquiline and Linezolid) were considered in this study. DESeq2 was used to identify the differentially regulated genes. Novel genes which were up-regulated during antibiotic treatment were identified which could be antibiotic resistance factors. Further, to understand the resistance mechanism of the novel genes, we performed gene ontology and gene network analysis for the differentially up-regulated genes. Thus, the in-silicotranscriptome analysis paves way for a deeper understanding of the antibiotic resistance in M. tuberculosis. |
