| Autor: |
Ma, Qin, Fan, Zizhen, Wang, Ping, Ma, Siya, Wen, Jian, Cao, Fengqin, Lin, Xianwu, Yan, Rihui |
| Zdroj: |
Journal of Integrative Agriculture; March 2024, Vol. 23 Issue: 3 p938-947, 10p |
| Abstrakt: |
transformeris a switch gene for sex determination in many insects, which cooperates with transformer-2that is expressed in both sexes to regulate female differentiation, particularly in dipterans. Zeugodacus cucurbitae(Coquillett) is a very destructive pest worldwide, however, its sex determination pathway remains largely uncharacterized. Here, we show that the female sex ratio is sharply reduced with knockdown of either transformeror transformer-2by RNA interference in early embryos of Z. cucurbitae. Most of the males grown from the embryos with transient transformerand transformer-2suppression mated with wild-type females and produced mixed sex progeny, with one exception that produced only female progeny, and all of the few remaining males failed to mate with wild-type females and produced no progeny. The exceptional male and those males with mating failure were XX pseudomales as determined by the detection of Y chromosome-linked Maleness-on-the-Y, indicating that most XX pseudomales are not viable. The phenotypes of transformerand transformer-2suggest that they play a key role in regulating sex determination and are required for female sexual development of Z. cucurbitae. Our results will be beneficial to the understanding of sex determination in Z. cucurbitaeand can facilitate the development of genetic sexing strains for its biological control. |
| Databáze: |
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