Exponential Equation Describing Kinetic Process of Immobilized β-Amylase Extracted from Glycine maxSeeds

Autor: Lahmar, Imen, Radeva, Greta, Marinkova, Dessislava, Velitchkova, Maya, Yotova, Lyubov
Zdroj: Chemistry Africa; January 2024, Vol. 7 Issue: 1 p93-98, 6p
Abstrakt: Soybean (Glycine max), a widely recognized plant, exhibits diverse biological activities and contains several significant compounds that are utilized in traditional medicine. The objective of this study was based on the biochemical and molecular characterization of the extracted β-amylase. Identified enzyme was immobilized by adhesion to a specific matrix of Titanium dioxide-based hybrid materials composed of an organic polymer of cellulose acetate butyrate and a copolymer of acrylonitrile and acrylamide. Extracted β-amylase showed a molecular weight of 50 KDa. It was immobilized onto a mechanical stable matrix, flexible and resistant to abrasion. Promoted thermal stability was recorded at 70 °C. By studying the variation of conversion degree α with reaction time at different temperatures, the exponential equation best described the heterogeneous processes taking place on uniformly inhomogeneous surfaces. This novel expression holds practical significance as it determines the degree of hydrolysis required at predetermined temperature, the intended time and the initial enzyme activity. It can be useful to control the β-amylase hydrolysis of glucose during several sectors of food, industry and pharmaceutics.
Databáze: Supplemental Index