| Autor: |
Shetty, Arjun N., Rao, Srinath, Honnale, Havgeppa N., Parveda, Maheshwari, Modi, Rajasamarsen, Kavi Kishor, P. B. |
| Zdroj: |
Plant Physiology Reports; 20230101, Issue: Preprints p1-11, 11p |
| Abstrakt: |
Deembryonated cotyledons of Sesame (Sesamum indicumL.), an oil yielding crop plant, were transformed using Agrobacterium tumefaciensstrain LBA4404 with the binary vector pCAMBIA1301, having hygromycin resistance gene (hptII) as a selectable marker and β-glucuronidase (GUS) as a reporter and SbAPETALA37 (SbAP37) transcription factor (isolated from Sorghum bicolor) under the influence of the stress inducible promoter abscisic acid2 (ABA2) for salt stress tolerance (isolated from indica rice). Deembryonated cotyledons proved better explants compared to hypocotyls for shoot regeneration (data not shown). Green shoots were recovered from transformed explants on the Murashige and Skoog’s (MS) medium supplemented with 5 mg L−16-benzylaminopurine (BAP) + 1 mg L−1indole-3-acetic acid + 1 mg L−1ABA + 5 mg L−1silver nitrate (AgNO3) + 5 mg L−1hygromycin. Shoots (3–4 cm long) were transferred onto MS medium fortified with 3 mg L−1indole-3-butyric acid and 5 mg L−1hygromycin for rooting. Integration of the SbAP37and hptIIgenes was confirmed by amplifying them using polymerase chain reaction (PCR). Transgenic plants accumulated 2 to 3-folds higher proline under 150 mM NaCl stress when compared to untransformed control plants. Transgenic plants exhibited higher chlorophyll than the untransformed controls. While malondialdehye (MDA) levels were low, peroxidase (POX), superoxide dismutase (SOD) and catalase (CAT) activities were 2–3-folds higher in transgenics under NaCl stress. This work possibly paves the way for exploiting other candidate genes for engineering salt, drought and biotic stress tolerance in the recalcitrant and neglected orphan crop sesame. |
| Databáze: |
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