| Autor: |
Kumar, Sanjeev, Mondal, Kalyan K., Ghoshal, Thungri, Kulshreshtha, Aditya, Sreenayana, B., Lakshmi, M. Amrutha, Mrutyunjaya, S., Rashmi, E. R., Kalaivanan, N. S., Kundu, Aditi, Mani, Chandra |
| Zdroj: |
3 Biotech; July 2023, Vol. 13 Issue: 7 |
| Abstrakt: |
Bacterial panicle blight (BPB) caused by Burkholderia glumae(BG) has become significantly more prevalent in the rice-growing regions of North India. Based on virulence screening and in vitro quantification of toxoflavin, the BG strains were classified as hyper- (BG1 and BG3), moderate- (BG2, BG4, BG6, BG8, and BG9), and hypo- (BG5, BG7, and BG10) virulent. Plant inoculation assays with cell-free culture filtrate revealed strains with higher toxoflavin-producing ability had higher virulence. Based on 16S rRNA sequence, 6 isolates from Uttar Pradesh were grouped in clad C1; whereas, clad C2 exhibited 4 isolates, two each from Delhi and Uttar Pradesh. Strain BG1 being the most virulent Indian strain from Uttar Pradesh was further profiled for 11 toxgenes. We found all the 11 toxgenes present in strain BG1. In toxRABCDEcluster, all toxgenes showed high similarity to B. glumaeBGR1 except toxB, whereas in toxFGHIJcluster toxF, toxG, toxHand toxIshared maximum similarity to B. glumae336gr-1. toxgenes of BG1 exhibited homology as well as divergence with B. gladioli. The domain prediction and protein association network analysis indicated the possible involvement of toxgenes in the toxoflavin biosynthesis. As per our knowledge, this is the first report in India on characterization of toxgenes cluster in B. glumae. Altogether, our study unravels a reliable method for identifying and characterizing B. glumaeusing toxgenes and its relationship with disease production. |
| Databáze: |
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