| Autor: |
Werby, Sabrina H., Bri, Jasna, Chosy, Madeline B., Sun, Jiuzhi, Rendell, Jacob T., Neville, Lewis F., Wender, Paul A., Cegelski, Lynette |
| Zdroj: |
MedChemComm; 2023, Vol. 14 Issue: 6 p1192-1198, 7p |
| Abstrakt: |
The introduction of new and improved antibacterial agents based on facile synthetic modifications of existing antibiotics represents a promising strategy to deliver urgently needed antibacterial candidates to treat multi-drug resistant bacterial infections. Using this strategy, vancomycin was transformed into a highly active agent against antibiotic-resistant Gram-negative organisms in vitroand in vivothrough the addition of a single arginine to yield vancomycin–arginine (V–R). Here, we report detection of the accumulation of V–R in E. coliby whole-cell solid-state NMR using 15N-labeled V–R. 15N CPMAS NMR revealed that the conjugate remained fully amidated without loss of arginine, demonstrating that intact V–R represents the active antibacterial agent. Furthermore, C{N}REDOR NMR in whole cells with all carbons at natural abundance 13C levels exhibited the sensitivity and selectivity to detect the directly bonded 13C–15N pairs of V–R within E. colicells. Thus, we also present an effective methodology to directly detect and evaluate active drug agents and their accumulation within bacteria without the need for potentially perturbative cell lysis and analysis protocols. |
| Databáze: |
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