| Abstrakt: |
Very few researchers have focused on the biological efficacy of Limoniumplants. In this concern, no investigations were commenced to delve into the in vitro and ex vivo biological actions of Limonium pruinosumin Egypt. Therefore, this work aims to assess for the first time the antimicrobial, antioxidant, and antitumor activities of Limonium pruinosumextract in addition to studying its ability to suppress the transcription of cell cycle–stimulating genes. L. pruinosumethyl acetate extract exhibits considerable antibacterial and antibiofilm activity versus E. coliATCC 25922 and Staphylococcus aureusATCC 25923. Results revealed that L. pruinosumexerts antioxidant effectiveness concerning DPPH, nitric oxide (NO), and hydroxyl radical (OH) scavenging ability with an IC50(35.88 ± 2.2, 51.31 ± 1.06, and 65.87 ± 1.19 μg/mL) respectively. The results proved the effectiveness of L. pruinosumin closing wounds in gastric epithelial cells (GES-1) by (79.9343 ± 1.98%) compared with control (68.3637 ± 2.32%) in 48 h. Additionally, L. pruinosumhad anticancer activity contrary to breast cancer MCF-7 and liver cancer HepG-2 cell lines with IC50values of 96.73 ± 2.18 and 81.81 ± 0.99 μg/mL, respectively, while it had no cytotoxic activity against (Wi-38) normal cells. Also, L. pruinosumextract provoked considerable early- and late-apoptotic cell populations and was effective in inducing cell death of MCF-7. Our findings evoked that L. pruinosumhas promising antibacterial, antioxidant, and wound healing activities and a good breast tumor suppressor arresting the cell cycle-stimulating genes, which may be an auspicious approach for the treatment of breast cancer. |
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