| Autor: |
Tao, Leyuan, Wang, Bing, Xin, Shichao, Li, Wei, Huang, Shengcai, Liu, Laihua, Cui, Jing, Zhang, Qianru, Cheng, Xianguo |
| Zdroj: |
The Crop Journal; 20230101, Issue: Preprints |
| Abstrakt: |
Aquaporins play important regulatory roles in improving plant abiotic stress tolerance. To better understand whether the OsPIP1genes collectively dominate the osmotic regulation in rice under salt stress, a cluster editing of the OsPIP1;1, OsPIP1;2and OsPIP1;3genes in rice was performed by CRISPR/Cas9 system. Sequencing showed that two mutants with Cas9-free, line 14 and line 18 were successfully edited. Briefly, line 14 deleted a single C base in both the OsPIP1;1and OsPIP1;3genes, and inserted a single T base in the OsPIP1;2gene, respectively. While line 18 demonstrated an insertion of a single A base in the OsPIP1;1gene and a single T base in both the OsPIP1;2and OsPIP1;3genes, respectively. Multiplex editing of the OsPIP1genes significantly inhibited photosynthetic rate and accumulation of compatible metabolites, but increased MDA contents and osmotic potentials in the mutants, thus delaying rice growth under salt stress. Functional loss of the OsPIP1genes obviously suppressed the expressions of the OsPIP1, OsSOS1, OsCIPK24and OsCBL4genes, and increased the influxes of Na+and effluxes of K+/H+in the roots, thus accumulating more Na+in rice mutants under salt stress. This study suggests that the OsPIP1genes are essential modulators collectively contributing to the enhancement of rice salt stress tolerance, and multiplex editing of the OsPIP1genes provides insight into the osmotic regulation of the PIPgenes. |
| Databáze: |
Supplemental Index |
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