Prevalence and genetic diversity of Theileria equifrom horses in Xinjiang Uygur Autonomous region, China

Autor: Zhang, Yang, Shi, Qianyun, Laven, Richard, Li, Caishan, He, Wenwen, Zheng, Huizhen, Liu, Shifang, Lu, Mingmin, Yang, Danchen Aaron, Guo, Qingyong, Chahan, Bayin
Zdroj: Ticks and Tick-borne Diseases; July 2023, Vol. 14 Issue: 4
Abstrakt: Theileria equiis a tick-borne intracellular apicomplexan protozoan parasite that causes equine theileriosis (ET). ET is an economically important disease with a worldwide distribution that significantly impacts international horse movement. Horses are an essential part of the economy in Xinjiang which is home to ∼10% of all the horses in China. However, there is very limited information on the prevalence and genetic complexity of T. equiin this region. Blood samples from 302 horses were collected from May to September 2021 in Ili, Xinjiang, and subjected to PCR examination for the presence of T. equi. In addition, a Bayesian latent class model was employed to estimate the true prevalence of T. equi,and a phylogenetic analysis was carried out based on the 18S rRNA gene of T. equiisolates. Seventy-two horses (23.8%) were PCR positive. After accounting for the imperfect PCR test using a Bayesian latent class model, the estimated true prevalence differed considerably between age groups, being 10.8% (95%CrI: 5.8% - 17.9%) in ≤ 3-year-old horses and 35.7% (95%CrI: 28.1% - 44.5%) in horses that were > 3 year-old. All T. equiisolates had their 18S rRNA gene (430bp) sequenced and analyzed in order to identify whether there were multiple genotypes of T. equiin the Xinjiang horse population. All of the 18S rRNA genes clustered into one phylogenetic group, clade E, which is thus probably the dominant genotype of T. equiin Xinjiang, China. To summarize, we monitored the prevalence of T. equiin horses of Xinjiang, China, with a focus on the association between age and the occurrence of T. equiby Bayesian modelling, accompanied by the genotyping of T. equiisolates. Obtaining the information on genotypes and age structure is significant in monitoring the spread of T. equiand studying the factors responsible for the distribution.
Databáze: Supplemental Index
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