| Abstrakt: |
The cyanobacterium BTA 903 isolated from Manipur state of India was identified as Anabaenasp. after phylogenetic analysis. BTA 903 contained ash (5.84% w/w), carbohydrate (42.36% w/w), protein (33.46% w/w), lipid (16.46% w/w), and moisture (1.88% w/w), respectively. BTA 903 required 0.3 M glycine and 5 mM sodium glutamate as supplements in BG 11 media in order to achieve the highest amount of cell growth along with C-phycoerythrin (PE) production. The light intensity of 6 k Lux, along with a photoperiod of 14:10 (L:D) was revealed to be optimal for increased productivity. The yields of C-phycoerythrin, C-phycocyanin, allophycocyanin, chlorophyll-a, and ammonia excretion after 30 days of incubation of Anabaena sp. were 49.96, 27.53, and 30.39, 6.9, and 31.2 mg per gram of biomass, respectively. The biomass productivity of Anabaenasp. BTA 903 was found to be 39 mgL−1day−1after 30 days of incubation. PE from Anabaenasp. was extracted using 0.05 M phosphate buffer (pH-7). The deactivation process of PE was investigated using a first-order kinetic model. The rate constant (kd) was found to be in the range of 0–0.01 h−1when the deactivation study was carried out at pH 7 and temperatures ranging from 10 to 30 °C. The highest level of PE deactivation was found at 80 °C and pH 12 due to a higher kdvalue (0.671 h−1). At pH 7, the highest Gibb’s free energy for PE was found to be 103.70–115.69 kJ/mol. PE’s pH and thermal tolerances suggested that it could be used for industrial applications. |
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