| Autor: |
Abd Elghani, Eman M., Elsayed, Abeer M., Omar, Farghaly A., Abdel-Aziz Emam, Marwa M., Tadros, Soad H., Soliman, Fathy M., Al-Mahallawi, Abdulaziz M. |
| Zdroj: |
Journal of Essential Oil Bearing Plants; January 2023, Vol. 26 Issue: 1 p190-205, 16p |
| Abstrakt: |
AbstractPeptic ulcer associated with H. pyloriinfection and antibiotics resistance warranted the execution of the foregoing study to produce natural and safe alternatives. GC-MS analysis of the essential oil derived from the ripe and unripe peel of Citrus sinensisvar. Valencia grown in Egypt was investigated besides its anti-Helicobacter pyloriand COX-2 inhibition actions. Ripe mature peels displayed a higher oil yield than unripe ones (0.8 and 0.6%, respectively). Twenty-nine compounds were identified in all samples with volatile monoterpenes as the predominant class, (88.90% and 90.62% in fresh ripe peel (P1) and dried ripe peel (P2), respectively, however unripe fresh orange peel (P3) showed a lower quantity (83.95%). D-Limonene was the major compound in P1 and P2 (85% and 88 %, respectively), while 73% were found in P3. In comparison to standard Clarithromycin (MIC90 = 1.95 ± 0.002 μg/ml), the nano-hexosomal formula of P1 reduced H. pyloriaction more than P1 alone with MIC90= 1.95 ± 0.0029 μg/ml and MIC90= 3.90 ± 0.006 μg/ml for formulated and unformulated P1, respectively. An anti-inflammatory assay viaCOX-2 inhibition revealed that the formulated P1 is more potent (IC50= 0.85 μg/ml) than P1 alone (IC50= 2.04 μg/ml) in comparison with Celecoxib® (IC50= 0.28 μg/ml). To unravel the mechanism of the anti-Helicobacter pyloriactivity, an in silicostudy on D-Limonene has been performed, using H. pyloriurease enzyme, which revealed that D-Limonene showed promising binding scores. Thus, Valencia orange peel essential oil offers a natural, cheap, and relatively safe candidate to combat Helicobacter pyloriinfection. |
| Databáze: |
Supplemental Index |
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